Barrientos A The Mitoribosome Methods and Protocols 2023

Barrientos A The Mitoribosome Methods and Protocols 2023 | 11.23 MB
English | 346 Pages

Title: The Mitoribosome
Author: Antoni Barrientos
Year: 2023

Description:
This volume provides classic and new methods to study the structure, assembly pathway, and protein synthesis ability of mitoribosomes across species.  Following an introduction of fundamental concepts on the topic, method chapters present detailed protocols based on cryo-electron tomography, cryo-EM approaches, mitoribosome purification techniques, mitochondrial translation assays, and methods to study mitochondrial mRNAs that are translated on mitoribosomes. Written in the format of the highly successful  Methods in Molecular Biology  series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge,  The Mitoribosome: Methods and Protocols,  aims to be a comprehensive guide for researchers in the field.

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